Research Areas
Email: Teerapong.B@chula.ac.th Phone: 662-218-5436
Teerapong Buaboocha, Associate Professor, Ph.D.
Omics research of interest Genomics and transcriptomics analysis of the mechanisms of stress responses in rice. Current focuses are on using genome-wide association study to identify salt-tolerant genes from local Thai rice and transcriptome profiles to understand the mechanisms of calcium-mediated responses to salt stress. Because stress responses are mediated by complex networks of cell signaling, understanding mechanisms of how plants perceive and transmit stress signals and how the functional and regulatory proteins contribute to the tolerance require knowledge of a genome scale. Currently, we have sequenced the exomes of nearly 200 local Thai rice varieties using the next generation sequencing. The exome was captured using capture probes designed based on all rice genes and the promoters of salt stress related and responsive genes. The resulting genotypes are associated with various phenotypes relating to salt stress responses to identify salt-tolerant genes. The second omic approach that we use is transcriptomics analysis to generate comparative transcriptome profiles between rice or Arabidopsis overexpressing genes of interest when grown under salt stress. Currently, we are working on a family of calcium -binding proteins, calmodulin (CaM) in rice and trying to determine whether and how they contribute to stress tolerance. Selected publications 1. Chinpongpanich A, Phean-o-pas S, ThongchuangM, Qu Li-Jia, Buaboocha T. C-Terminal Extension of Calmodulin-Like 3 Protein from Oryza sativa L.: Interaction with a High Mobility Group Target Protein. Acta Bioch Bioph Sin 2015, 47: 880-889 2. Udomchalothorn T, Plaimas K, Comai L, Buaboocha T, Chadchawan S. Molecular karyotyping and exome analysis of salt-Tolerant rice mutant from somaclonal variation. Plant Genome 2014, 7:3 3. Seang-Ngam S, Limruengroj K, Pichyangkura R, Chadchawan S, Buaboocha T. Chitosan potentially induces drought resistance in rice Oryza sativa L. via calmodulin. J Chitin Chitosan Sci, 2014, 117-122 4. Sripinyowanich S, Chamnanmanoontham N, Udomchalothorn T, Maneeprasopsuk S, Santawee P, Buaboocha T, Qu L-J, Gu H, Chadchawan S. Overexpression of a partial fragment of the salt-responsive gene OsNUC1 enhances salt adaptation in transgenic Arabidopsis thaliana and rice (Oryza sativa L.) during salt stress. Plant Science, 2013, 213: 67-78 5. Sripinyowanich S, Klomsakul P, Boonburapong B, Bangyeekhun T, Asami T, Gu H, Buaboocha T, Chadchawan S. Exogenous ABA induces salt tolerance in indica rice (Oryza sativa L.): the role of OsP5CS1 and OsP5CR gene expression during salt stress. Environ Exp Bot 2013, 86: 94-105 6. Chinpongpanich A, Limruengroj K, Phean-o-pas S, Limpaseni T, Buaboocha T. Expression analysis of calmodulin and calmodulin-like genes from rice, Oryza sativa L. BMC Research Notes 2012, 5:625 7. Saeng-ngam S, Takpirom W, Buaboocha T, Chadchawan S. The role of the OsCam1-1 salt stress sensor in ABA accumulation and salt tolerance in rice. J Plant Biol 2012, 55: 198-208 8. Chinpongpanich A, Wutipraditkul N, Thairat S, Buaboocha T. Biophysical characterization of calmodulin and calmodulin-like proteins from rice, Oryza sativa L. Acta Bioch Bioph Sin 2011, 43: 867-876
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