ORDERING

Sample submission

Please bring your order form and samples to the Omics center at 12th floor Maha-Vajirunhis building (9:00 am - 5:00 pm, Monday - Friday). All samples should meet the following criteria: Starting DNA/RNA material MUST meet concentration listed below. Please dilute your samples if they are out of range.

• Use fluorometric based methods for quantification (Qubit or PicoGreen) of the template instead of UV spec based methods (e.g., NanoDrop) to obtain accurate DNA measurement.
• DNA should have absorbance ratio values of 1.8–2.0.
• RNA samples can be suspended in RNase-free water water.
• RNA integrity should be assessed using the Agilent Bioanalyzer (or any similar system).
• Submit samples in a 0.2mL PCR tube.
• Label tubes with sample name.
• Samples should be on dry ice.

For Ready-to-run libraries: Please provide ≥20 µl of your ready to run library at a 10 nM concentration in 10 mM Tris pH 8. If you would like to use a custom primer for your MiSeq run, please submit ≥40 µl at a concentration of 10 mM. Please add any QC information (Qubit data and/or Agilent run, qPCR) available to the online order form.

We run QC assays on all samples submitted for Next Generation Sequencing. Our staff will give you recommendations for deciding if you should run or not run your sample. This decision is based on expected cluster density and potential contaminating sequences or lack of insert sequence.

Alternatively, you can run a formaldehyde 1% agarose gel and judge the integrity of RNA upon staining with ethidium bromide.
• High-quality RNA shows a 28S rRNA band at 4.5 kb that should be twice then intensity of the 18S rRNA band at 1.9 kb.
• Both kb determinations are relative to an RNA 6000 ladder.
• The mRNA appears as a smear from 0.5 kb to 12 kb.

In most instances, data can be shared with our customers though BaseSpace, a free online service provided by Illumina. Sharing projects though BaseSpace allows for immediate transfer and unlimited cloud storage of sequencing run data. Customers who utilize this service will have indefinite access to their sequencing runs, and access to project results can be shared to collaborators at the owner’s discretion. You can register for an account and access this free and secure service at https://basespace.illumina.com. Alternatively, data can be downloaded onto a hard drive.

Important note The DNA integrity is highly critical for preparing library that contains deep coverage and great complexity of the genome. Therefore, we recommend checking the DNA integrity on a 0.7% TAE Agarose gel before submission. DNA should be diluted in DNase-free water or law-salt, weakly buffered solutions containing little or no metal ion chelating agents such as EDTA. The quality of starting RNA is crucial for making good libraries and the most important for robust data analysis. The starting material should be within the range of 0.1-4 µg of intact total RNA (RIN>8). In addition, RNA samples can be suspended in RNase-free water. RNA integrity should be assessed using the Agilent Bioanalyzer (or any similar system). We highly recommend using the fluorometry for more accurate DNA and RNA quantification in your projects.

Bioanalyzer Quality Check Request Form (contact us)

Fluorometer Quality Check Request Form (contact us)

RNA library prep submission form (contact us)

DNA library prep submission form (contact us)

Raw sample submission form
(contact us)

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